In contrast, shoots cultured on solidified media containing oryzalin failed to produce polyploid nuclei suggesting limited uptake and/or transport of oryzalin in shoot cultures. Using aqueous treatments of oryzalin and shoot cultures, polyploid C. glabra plants were successfully produced, rooted, and established in the greenhouse. Although an increase in the frequency of polyploid nuclei was detected in callus cultures grown on media containing orzyalin, these treatments significantly reduced callus growth and viability.