The postharvest spoilage of Guava fruit (Psidium guajava Linn.) from four selected markets in Awka, Anambra State, Nigeria was investigated. Isolation of associated fungi from guava fruits was carried out on Sabouraud dextrose agar (SDA). A total of seven (7) fungi were isolated namely Colletotrichum gloeosporioides, Fusarium oxysporum, Mucor sp., Rhizopus stolonifer, Aspergillus niger, A. fumigatus and A. parasiticus.