On the bottom of each agar plate (140 mm in diameter), a circle of 90 mm in diameter was drawn. Then 450 μL of a diluted bacterial culturewas spread on each agar plate within the circle
On the bottom of each agarplate (140 mm in diameter), a circle of 90 mm in diameter was drawn.Then 450 μL of a diluted bacterial culturewas spread on each agar platewithin the circle