2.4. Microbiological enumeration
A 0.1 ml sample was taken from a TDT disk and serially
diluted in sterile Butterfield’s phosphate buffer (pH 7.0,
Hardy Diagnostics, Santa Maria, CA) with a minimum
1 ml transfer. Duplicate samples were then pour plated
with TSA and the plates incubated at 37 C for 24 h. Plates
with 30–300 colonies were enumerated using a manual colony
counter (Bantex, Burlingame, CA, Bantex Colony
Counter 920).