Carrot roots were sliced and frozen at –30 ◦C for subsequent analysis of soluble solids, carotenoids and ascorbic acid content. On the day of analysis root were kept out of freezer to obtain juice. The extracted juices were collected in 50ml volumetric flask. Further root samples were mashed inmortar and pestle for juice extraction and then the collected juice were mixed, and analyzed for soluble solid content. About 0.4ml of the mixed juice was placed onto the prism surface of pocket digital refractometer (PAL-1, Atago Ltd., Tokyo, Japan) and soluble solid contents were recorded. Repeated measures were conducted bywashing the prismsurface by distilled
water and also rinsed with the test juice.