The PCR was performed at 94 C for 1 min; 35 cycles of 94 C for 20 s, 53 C for 25 s, and 68 C for 45 s; and a final extension at 68 C for 10 min using the AccuPrime High Fidelity Taq Polymerase (Invitrogen, Grand Island, NY, USA). The
The PCR was performed at 94 C for 1 min;35 cycles of 94 C for 20 s, 53 C for 25 s, and 68 C for 45 s; anda final extension at 68 C for 10 min using the AccuPrime HighFidelity Taq Polymerase (Invitrogen, Grand Island, NY, USA). The