2.5. Chlorophyll measurement
Chlorophyll content was measured in leaf frozen samples (10–
20 mg FW) which was extracted one day in 1 mL of methanol. The
absorbance of methanolic extracts was measured at 470, 652.4 and
665.2nmusing UVmini 1240 Shimadzu spectrophotometer (Shimadzu
Scientific Instruments, USA). The chlorophyll and carotenoid contents
were calculated by a formula according to Lichtenthaler (1987).