2. Materials and methods
2.1. Animals
Live wild sandworms (average weight 2.73 g/worm) were obtained from Bangphra beach, Chonburi province, Thailand,
in August 2006 and starved for 2 days before putting them on ice and freezing at −70 ◦C. Four-month-old farmed sandworms
were obtained from a commercial sandworm farm in Samutsongkram province (average weight 0.73 g/worm). The farmed
sandworms were fed with commercial shrimp diet (containing protein 380 g/kg, fat 50 g/kg and fiber 40 g/kg). Upon arrival,
farmed sandworms were starved for 2 days to clear the digestive tract before putting them on ice and then frozen at
−70 ◦C
until used