The crude extract which showed maximum antibacterial and antioxidant activities (Ethanol extract) was subjected to fractionation by silica gel G 60-120 mesh column. Ethyl acetate has been used as mobile phase for gradient elution. Elutes were detected and separated simply using UV absorption (254 nm) and TLC spot observation (Ethyl acetate, Methanol, Water, 5:4:1). Each of the eluate of ethanol extract was again subjected to antibacterial and antioxidant activities.