External primers were: 5’-TAAACAAACACACACTCTAAGG-
3’ and 5’-
CATCTGATTGAAGTGGAC-3’, and internal
primers were: 5’-GGTTCACGCATACGTTAAGCCTCACTT-
3’ and 5’-TATCACTAGACAATAACCGGATGGGTA-
3’ (Olmos et al. 1999). The amplification
product (131 bp) was detected in a 0.8% agarose
gel stained with ethidium bromide.