Combination of plant growth regulator also had successfully induced the formation of callus culture from many higher plants. Leonurus heterophylus callus culture was induced on ms medium supplied with combination of BAP and 24d Nirmalakumari also reported on callus induction from bark of Vitex negungob on medium supplemented with combination 24d and kinetin .The fotmation of callus from Vigna mungo explant was induced on media containing BAP and 24d