From Figure A and B the growth of Escherichia coli and Klebsiella oxytoca concluded that from Figure 3-A Escherichia coli with no food supplement. Over the culture time , there will be more than Klebsiella oxytoca optical density and are more likely than constant optical density. When I put the 0.5% yeast extract, Between 0-20 hours Escherichia coli is optical density increased rapidly after that, regularly, but will gradually increase the time Klebsiella oxyoca 0-20 hours to have density increases rapidly afterwards will gradually decrease regularly. When they 0.5% yeast extract, thiamine and micronutrients at the time of 0-20 hours.D is density increased rapidly thereafter, the density is not constant, but e time 0-20 h to have density increases rapidly after that density is constant