Abstract
The enzyme cellulase is produced by utilizing contemptible substrates like rice bran and corn straw supplanted with nutrient sources in Solid Fermentation using the fungal strain Trichoderma reesei (MTCC *164). A fermentation time of 6 day is required to obtain maximum Cellulase and β-glycosidase activity. The activity decreases with further increase in fermentation period. The optimum rice bran- corn straw ratio, pH and temperature for maximum production of Cellulase are found to be 5:5,5and28C respectively. The crude enzyme is partially purified by Ammonium sulphate Precipication,Dialysis and finally Ion Exchange Chromatography. The effect of temperature,pH and substrate concentration were also studied in the purified sample and the optimum condition for enzyme activity is found to be pH 5.0,temperature 50C.