Lipase characterization:
Polyacrylamide gel electrophoresis: Enzyme
purification or purity was checked on non
denaturing native PAGE using 10% gel
concentration with slight modification on method
described by Holt and Hartman (1994). Gel was
stained using the Coomassie Brilliant Blue R-250
staining solution. Total enzyme protein used for
PAGE was of 10 μg for crude as well as partially
purified lipase