In our study, 10 CFU of tested microorganisms
per gram of food sample was inoculated, and after 5–7 h enrichment,
1–2 × 103 cells could be used for 25 μl volume of real-time
PCR reaction. For the antibody-based lateral flow strip test, we had to
incubate up to 6–8 h for the detectable positive band on the strip, and
the cell numbers were estimated to be between 105–106 CFU/ml.