Most of the current tissue culture methods are based on technologies developed over twenty years ago. The commercial use of plant tissue culture primarily involves the production of large number of plants with minimum input expenses. The main factors which ultimately influence the commercial propagation of plants in vitro are: the selection of plant species, the physical environment and the chemical media for in vitro culture. Research efforts have addressed growth regulating
compounds and salt mixes and there seems to be good scope for substituting the expensive chemical nutrient media with low cost natural extracts.