For extraction of grinded sunflower seeds with subcritical water
a 60 mL cylindrical stainless steel high-pressure batch extractor
(Autoclave Engineers, USA) was used. Temperature regulation
was performed with a heating cable and stirring of the extraction
media was performed by using a magnetic stirrer. The filled extractor
was purged three times with inert nitrogen gas to remove present
atmospheric oxygen, which could cause oxidation of oil during
extraction. The applied extraction pressure for all extractions was
equal to 30 bar and was held constant throughout the extraction.