Olivera and Salvadori (2009), suggested that slow freezing formed large size ice crystals causing the disruption of dough gluten network during freezing. Meziani et al. (2012) shown the dependence of fermentation activity and integrity of the gluten network with freezing rate, which controls size and location of ice crystals resulting in research of a compromise between freezing rate nor too fast to reduce yeast viability, nor too slow to form large ice crystals that could perforate gluten network.