tThe aim of this study was to determine how dietary supplementation of inorganic andorganic selenium affects the selenium concentration and glutathione peroxidase activityin blood and sperm of sexually mature stud boars. Twenty-four boars of the Large White,Landrace, Pietrain and Duroc breeds of optimal breeding age (on average 2.5 years old)were used. The study lasted 90 days. The boars were randomly assigned to one of threedietary treatment groups: T1 = control; no added selenium (n = 8 boars), T2 = added 0.3 ppminorganic selenium (sodium selenite, Microgran®Se 1% BMP) (n = 8 boars), and T3 = added0.3 ppm organic selenium (Se-yeast, Sel-Plex 2000®) (n = 8 boars). The concentration ofselenium was determined in whole blood and semen, while the activity of glutathione per-oxidase (GPx) was measured in blood plasma and semen. In order to measure GPx activityin semen, reactivation of the enzymatic GPx activity was performed. The determined sele-nium concentration in blood was lowest in the non-supplemented group of boars. Bloodplasma GPx activity was higher in boars fed organic selenium than in boars fed a diet with-out supplemented selenium. While the supplementation of sodium selenite significantlyincreased GPx activity in boar semen. The highest-concentration of selenium in semen atthe end of the trial was determined in the group of boars supplemented with organic sele-nium, somewhat lower in boars fed supplemented inorganic selenium, and the lowest inthe non-supplemented group of boars. The only significant difference between the seleniteand Se-yeast diet supplementation was observed in the Se concentration of the semen. Thesupplementation of selenium affected semen quality, and organic selenium improved theprogressive motility of the spermatozoa and increased their resistance in hypo-osmotic andthermal tests. The storage ability of short term preserved semen was improved by organicselenium supplementation, as well as also increasing the fertility rate in gilts.
tThe aim of this study was to determine how dietary supplementation of inorganic andorganic selenium affects the selenium concentration and glutathione peroxidase activityin blood and sperm of sexually mature stud boars. Twenty-four boars of the Large White,Landrace, Pietrain and Duroc breeds of optimal breeding age (on average 2.5 years old)were used. The study lasted 90 days. The boars were randomly assigned to one of threedietary treatment groups: T1 = control; no added selenium (n = 8 boars), T2 = added 0.3 ppminorganic selenium (sodium selenite, Microgran®Se 1% BMP) (n = 8 boars), and T3 = added0.3 ppm organic selenium (Se-yeast, Sel-Plex 2000®) (n = 8 boars). The concentration ofselenium was determined in whole blood and semen, while the activity of glutathione per-oxidase (GPx) was measured in blood plasma and semen. In order to measure GPx activityin semen, reactivation of the enzymatic GPx activity was performed. The determined sele-nium concentration in blood was lowest in the non-supplemented group of boars. Bloodplasma GPx activity was higher in boars fed organic selenium than in boars fed a diet with-out supplemented selenium. While the supplementation of sodium selenite significantlyincreased GPx activity in boar semen. The highest-concentration of selenium in semen atthe end of the trial was determined in the group of boars supplemented with organic sele-nium, somewhat lower in boars fed supplemented inorganic selenium, and the lowest inthe non-supplemented group of boars. The only significant difference between the seleniteand Se-yeast diet supplementation was observed in the Se concentration of the semen. Thesupplementation of selenium affected semen quality, and organic selenium improved theprogressive motility of the spermatozoa and increased their resistance in hypo-osmotic andthermal tests. The storage ability of short term preserved semen was improved by organicselenium supplementation, as well as also increasing the fertility rate in gilts.
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