TRH was prepared by culturing T. rubrum in Saboroud dextrose agar for 10–14 days. Fungal mycelia were collected and dried at 50 ℃ overnight. Dried mycelia (150 mg) were then finely pulverized, and suspended in 20 ml of B5 medium. The suspension of the pulverized mycelia was autoclaved at 121 ℃ for 15 min and diluted with distilled water to various concentrations (Table 1) prior to use (Al-Gendy and Lockwood, 2005).
YE was suspended in B5 medium at pH 5.5 ± 0.1, to afford a stock solution of 0.3 g/ml and sterilized by autoclaving at 121 ℃ for 15 min and adjusted to various elicitor concentrations (Table1) prior to use (Al-Gendy and Lockwood, 2005).
CHI was dissolved in distilled water. The solution was adjusted to pH 5.5 with 1 N NaOH, and the final concentration, adjusted to 10 mg/ml. Aliquot was autoclaved for 15 min at 121 ℃ prior to use. MJ was dissolved in 95% (v/v) ethanol. The solution (30 mM) was filtered through a microfilter (0.22 lm) prior to use (Hwa-Young et al., 2008).