In order to perform PCR test, methods of isolating DNA from
purified bacteria and filtered water samples were first used in
this study. Then, with the aim of accelerating and simplifying
the test procedure in the routine implementation, mass PCR was
performed. In mass PCR method, cell mass obtained from water
samples was used directly after filtration and concentration.Final volume of PCR was considered as 25 mL, containing 20 mL of all PCR components together with 5 mL of microbial biomass.
To avoid any errors, PCR reaction for pure colonies of
isolated bacteria were conducted in the same method.
Colonies dissolved in 250 mL distilled water, and filtrated and
concentrated biomass used in PCR reaction.