The exogenous hormones applied during the second experiment proved to be effective for growth and development of cotton root. All the parameters measured in the first cropping without hormones application and second cropping with hormone application were insignificant except for percentage active absorption area which was negatively correlated (-0.999*).This result was similar to Tao et al., (1993) on pea seedling growth, Chunrong et al., (2008) on endogenous and exogenous hormones of water stress in maize seedling, Zahir et al., (2001) on growth and yield of rice, Kerbauy and Colli (1997) on root of C. fimbriatum.