Temperature of water in water jars and metal containers associated with DHF patients in this study. The result corresponded with a research of Wongkoon et al. [12] and Lester et al. [8]. They founded that the mean temperatures, container surface area and water depth influenced the population dynamics of the mosquito and the transmission of dengue disease. As the mean temperature increased, the DHF case also increased. It was possible that most of the physiological functions of vectors in this area were subject to optimal mean temperature. Opened water-filled containers and number of Aedes larvae, temperature of water in water jar and metal container displayed the association with DHF patients in Kreang sub district, Cha-uat district Nakhon Si Thammarat. If we can estimate the number of Aedes positive containers or Aedes larvae, we can predict the DHF patients each household. Provincial Health Office may have to design a campaign for DHF prevention more specifically in each locality.