Abstract
Fodder additives using medicinal plants or plants extracts can be considered as a novel trend for control of
fish diseases and hoping to achieve the same results as in the use of antibiotics and to overcome the problem
of antibiotic resistance. The aim of our research is mainly focused to evaluate two different herbal additives
on growth performance, antioxidant and immune potentiating effects. Ropadiar powder plus® (Ropadiar)
(Oregano essential oil) and Jade Spirulina® (Spirulina platensis) were evaluated separately and used as feed
additives for cultured Nile tilapia whereas; a total of six treatments, i.e., negative control group (Fed on basal
diet without herbs), three groups fed on Spirulina diets (2.5 %, 5% and 10%) and others fed on Ropadiar
diets (5 % and 10%) to investigate their effects on the growth performance, body composition and serum
antioxidant activity. After eight weeks of feeding, all fish were experimentally challenged with pathogenic
Vibrio alginolyticus and mortalities were daily recorded for six days post infection. Results showed that fish
fed on 10% Ropadiar diet significantly enhanced the growth performance, promoted the deposition of
muscle protein and enhanced serum antioxidant activities of treated fish. Moreover, all fish groups fed on
diet with 10 % Spirulina and both concentrations of Ropadiar reduced the cumulative mortality percentage
(%) following infection, but the lowest mortality rate was observed in the group fed with 10 % Ropadiar
diet. It can be concluded that both tested Phytobiotics have an ideal growth promoting and immune
enhancing effects for cultured Nile tilapia and they can successfully replace the addition of antibiotics in fish
diets.
 
Abstract
Fodder additives using medicinal plants or plants extracts can be considered as a novel trend for control of
fish diseases and hoping to achieve the same results as in the use of antibiotics and to overcome the problem
of antibiotic resistance. The aim of our research is mainly focused to evaluate two different herbal additives
on growth performance, antioxidant and immune potentiating effects. Ropadiar powder plus® (Ropadiar)
(Oregano essential oil) and Jade Spirulina® (Spirulina platensis) were evaluated separately and used as feed
additives for cultured Nile tilapia whereas; a total of six treatments, i.e., negative control group (Fed on basal
diet without herbs), three groups fed on Spirulina diets (2.5 %, 5% and 10%) and others fed on Ropadiar
diets (5 % and 10%) to investigate their effects on the growth performance, body composition and serum
antioxidant activity. After eight weeks of feeding, all fish were experimentally challenged with pathogenic
Vibrio alginolyticus and mortalities were daily recorded for six days post infection. Results showed that fish
fed on 10% Ropadiar diet significantly enhanced the growth performance, promoted the deposition of
muscle protein and enhanced serum antioxidant activities of treated fish. Moreover, all fish groups fed on
diet with 10 % Spirulina and both concentrations of Ropadiar reduced the cumulative mortality percentage
(%) following infection, but the lowest mortality rate was observed in the group fed with 10 % Ropadiar
diet. It can be concluded that both tested Phytobiotics have an ideal growth promoting and immune
enhancing effects for cultured Nile tilapia and they can successfully replace the addition of antibiotics in fish
diets.
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