Mycelia plugs of the fungus
were grown on PDA medium at 25 C for three days and then inoculated
on straw decoction com (SDC) medium, incubated at 28 C
for 3 days, and transferred to a dark chamber for 5 days. We harvested
the fungus conidia using 7 ml sterile distilled water containing
0.5% gelatin and used to inoculate the plants. The suspension
was filtered through four layers of sterile cheesecloth, and
kept in a flask 4 C. Conidia concentration was measured using a
hemocytometer and adjusted to 1010 conidia/ml, before spraying.