2.4. Enzyme saccharification
Untreated and steam-exploded spent mushroom media, with
and without water extraction, were placed in 110-ml sample tubes at an initial substrate concentration of 3%(w/v) in 10 ml of 100 mM sodium acetate buffer (pH 5.0), using an enzyme (Meicelase; Meiji Seika Co. Ltd., Tokyo, Japan) loading of 0.1 g-enzyme/g-substrate