Many studies around the world have been conducted to find out the prevalence of FLA especially Acanthamoeba in hospital water sources. However, no such study has been performed in India. We present here the first study from India with the aim to screen for the presence of FLA in water sources used for drinking, bathing and hand washing by high risk patients admitted to bone marrow transplant (BMT), intensive care unit, high dependency unit (HDU) that caters to haemato-oncology patients, renal transplant intensive care unit (TICU) and haemodialysis unit (HU).