Data results on skin assessment show that there were no significant differences in baseline HSA among the 3 treatment groups. There were also no significant differences in baseline HSA among the periods or sequences. The change in HSA after 3 weeks and Method C, there was not a significant difference among the methods of surgical hand scrubs. Data results on microbial counts from each baseline data on each hand. By the end of the third week, Method C was associated with significantly lower microbial counts than Method B and A. There was no significant difference in baseline log counts among the 3 treatment groups. However, the reduction of microbiology hand washing after using each method was differs significantly. Data of comparing on the time and cost uses among three methods. There was a statistically significant time and cost saving method. Method C had significantly less time and cost compared to Methods A and B. There was no significant difference between Methods A and B for the cost and time.