The direct measurement of trypsin inhibitors has
been the most desirable assay to evaluate SBM quality
and processing conditions (Dozier and Hess, 2011).
Nevertheless, the analyses of trypsin inhibitors have
been time consuming, have required expensive equipment,
have produced inconsistent results probably due
to differences in methodologies between laboratories
(Rackis et al., 1974), and have been impractical for
day-to-day monitoring of SBM quality and adequacy
of heat treatment. Therefore, urease activity, PDI, and
KOH solubility analyses have become standard methods
to measure SBM quality.