A reversed-phase gradient elution system is described for the simultaneous separation of
the type I and type III isomers of 8-, 7-, 6-, 5- and 4-carboxylated porphyrins and
isocoproporphyrins. The method, adaptable for isocratic and stepwise separation of
individual groups of isomers, is also suitable for preparative isolation of pure porphyrins.
The analyses of porphyrin isomers in the urine and faeces of porphyric patients are
examples of applications.