recently reviewed several
methods of extending the antimicrobial spectrum of lysozyme to
Gram-negative bacteria, including denaturation of lysozyme, modification
by attachment of other compounds to lysozyme, and the
use of membrane-permeabilizing agents with lysozyme. A number
of studies have demonstrated that the antimicrobial spectrum of
lysozyme could be enhanced when it is used with other substances,
such as hydrogen peroxide and ascorbic acid (Miller 1969), ethylenediamine
tetra-acetic acid (EDTA) (Padgett and others 1998),
caffeic acid (Valenta and others 1998), and chitosan (Song and others
2001). The addition of lysozyme in a polymeric supporting matrix
by immobilization, absorption, or entrapment has been reported in
pharmaceutical and food-related areas.