The increasing incidence of multi-drug resistant A. baumannii and MRSA underscored the urgent need for effective alternative drug. The extraction of essential oil from plant is a crucial step for biosyn- thesis and may lead to the discovery of new drug for infectious diseases. This study revealed that all the multi-drug resistant bacteria were highly sensitive to makrut oil and makrut leaf oil. More than 80% of A. baumannii used in our study were resistant to all drugs tested in our routine clinical laboratory such as aminoglycosides, ampicillin, cephalosporins, carbapenem, fluoroquinolones; and only sensitive to colistin which is a drug with high nephro- and neuro- toxic potential. All MRSA in our study were hospital- acquired and resistant to most drugs available.