In doing so, several novel proteins were identified. In addition,
we present evidence for the existence of three dominant protein complexes
within the gastrolith, and identify the putative protein composition
of each suspected complex.We further found that GAP 65 is present
in each complex. On the basis of this last finding together with evidence
from immunostaining experiments detecting GAP 65 distribution and
localization in the gastrolith-forming epithelium (GFE) and gastrolith
matrix, we propose that GAP 65 serves as a central protein in the identified
protein complexes.