If you wish to use plants other than cauliflower you need to prepare two
different media which contain plant hormones necessary to stimulate
development of differentiated tissues. The first one should contain a cytokinin
such as BAP which promotes shoot formation and the second one a rooting
hormone such as NAA or store bought rooting hormone. To do this, prepare the
mixture up until the end of step 2. Keeping the mixture warm so that it does
not solidify, divide it equally into two pre-warmed containers. Each container
can be used to prepare 30 or so tubes as above. The first container should
have BAP added at the rate of 2.0mg/l. The second container should have the
NAA hormone added at the rate of 0.1 mg/L. To do this it is necessary to make
concentrated solutions of both BAP (2.0mg/ml) and NAA (1.0mg/ml) and filter
sterilize them. Add 1ml of the concentrated BAP stock or 100μl of the NAA
concentrated stock to each 1 liter of media that you prepare. If you use
rooting hormone that is purchased from your local hardware or nursery supply
store instead of NAA then just follow the directions before adding to your
media