Statistical analyses were performed using GraphPad Prism software
(GraphPad, San Diego, CA, USA). The sensitivity and specificity of ICT and
ELISA were defined with respect to MAT (used as the reference) and
were expressed as proportions with 95% confidence intervals (CIs). Positive
predictive value (PPV) was calculated as follows: number of true positives/(
number of true positives + number of false positives) × 100.
Negative predictive value (NPV) was calculated as follows: