During this method of spore morphological study Plates have to be prepared containing ISP2 medium. After solidification, by a sharp scalpel from the central portion of the plate, medium should be scooped out making a rectangular area. Then three sterile coverslips have to be placed on the hollow rectangular space. Slowly actinomycetes spores have to be inoculated at the edge of the coverslips touching the medium. The plates must be incubated at 28±2°C for 5 days and examined periodically taking out the coverslips.