In order to demonstrate that the strain could solubilize calcium Phytase in into available phosphorus without significant decrease in pH, a phytin agar plate test and an activity assay
of phytase and phosphatase were conducted. As shown in Fig. 2-A, a transparent zone appeared on screening agar medium containing calcium phytate after inoculated
with T. asperellum Q1 at 28ฐC for 3 d. Meanwhile, nothing appeared on the agar plate without T. asperellum Q1. Furthermore, activities of phytase and phosphatases in
Suspematant showed significant variations at different points of time after inoculation with T. asperellum Q1 comparing to uninoculated control. The highest phytase activity was
up to 0.17 U mL–1 (Fig. 2-B) on the 3rd day after inoculating T. asperellum Q1. The maximum of acid phosphatase activity was found to be 7.15ื10–3 U mL–1 after 2 d of incubation
whereas the alkaline phosphatase activity was much lower than that of acid phosphatase (Fig. 2-C). Therefore, all the results from in vitro phosphate solubilizing test, phytin agar
plate test and activity assay of phytase and phosphatase proved that metabolites (mainly enzymes) of T. asperellum Q1 could hydrolyze unsoluble organic phosphate into the
available phosphorus that was absorbed by strain Q1.