The precipitate was collected by centrifugation at 10,000 ×g for 30min at 4 °C, and the pellet was resuspended in 100 μl TE buffer and stored at −80 °C.
The precipitate was collected by centrifugation at 10,000 ×g for 30min at 4 °C, and the pellet was resuspended in 100 μl TE buffer and stored at −80 °C.