supplemented with fresh SD broth was inoculated with 1x107 cells/ ml P. aeruginosa 56402 (a clinical isolate obtained from the Microbiology Laboratory of Henry Ford Hospital in Detroit) in 6-ml polystyrene culture tubes (6 replications) and incubated on a gyratory shaker (170 rpm) at 35 °C for
24h. The growth inhibition of P. aeruginosa was determined spectrophotometrically by determining the optical density at 490nm and by colony forming unit (CFU) assay.