At baseline and after 90 days, facial wrinkles were measured in the crow's feet area using a skin replica and a Visiometer SV 600 (Courage+Khazaka Electronic, Köln, Germany). The Visiometer is a computerized instrument that makes a skin microrelief map from the replica using a light transmission method. It has 5 roughness parameters: depth of roughness (R1), mean depth of roughness (R2), maximum roughness (R3), depth of smoothness (R4) and arithmetic average roughness (R5).