2.7. Detection limit of oligonucleotide primers
The detection limit of all oligonucleotides as described in Table 1
was examined using Pig Genomic DNA (Novagen®, Germany). The
PCR assay condition was similar as described in the PCR amplification
using different oligonucleotide primers with different concentration
of porcine DNA ranging from 0 to 150 ng. While, in PCRsouthern
hybridization analysis, the condition used was similar as
described with porcine DNA but the concentration ranged from
0.00001 to 1 ng.