Pure cultures of C. gloeosporioides were maintained and further identification of this pathogen was conducted using the internal transcribed spacer region of the known C. gloeosporioides rDNA region 5.8S. Sequence similarity of the collected isolates was 100% with some C. gloeosporioides isolates and phylogenetic analysis also showed the close relationship. Although there are many prior reports on anthracnose disease on mango in India, confirmation of this disease on mango from isolates in India is confirmed by sequence analysis for the first time.