Removal of protein dissolved in water by batch foam separation was conducted with using
ovalbumin (OA) as a model protein in the light of wastewater treatment reducing organic
loading. The removal efficiency had a maximum value near the i.e.p. of OA (pH 4.6); thus,
most experiments were conducted at pH 4.6. Typical experimental conditions; superficial
gas velocity, Ug: 1.9710–2–5.3710–2 cm/s; initial bulk concentration of OA, Ci: ca.
0.05–0.25 g/L; liquid volume, V: 600cm3. A model estimating bulk concentration profile
was proposed by taking into account a mass balance of the present system. The model
predicted that OA could be removed perfectly, however, was not all removed experimentally.
The residual OA concentration of the bulk liquid within the column reached plateau
value, which correspond to ca. 18% of the initial OA concentration. The plateau value of the
bulk concentration was attained for ca. 100–500 min with Ug ¼ 1.9710–2—5.3710–2 cm/s.
Foaming ability test revealed that the foaming limit concentration of OA at pH 4.6 was
9.7210–3 g/L. These results suggested that OA molecules could be damaged by interaction
of bubble surface in the dispersed phase, since there were the residual OA concentrations
over the limit concentration. To take account of this phenomena and correct the model,
average surface density, Xd, which should convert protein molecule into the denatured
protein molecule, was introduced. The corrected model could explain well the time profile
of OA bulk concentration.