The antimicrobial activity of SBE-containing films was determined
by using the disc diffusion method. Discs of a diameter of 12 mm were
cut off the film (disc surface was c.a. 1 cm2) using a circular knife.
Inocula of bacteria were prepared by suspending indicatory cultures,
originating fromovernight cultures, inMHB at a quantity corresponding
to 0.5 McFarland (108 CFU/ml). Suspensions of yeasts and mold spores
at a concentration of 105 CFU/ml were prepared in physiological saline.
The suspensions of tested bacteria were spread evenly on the surface of
solidified MHA medium, and yeasts or mold spore suspensions on SA
medium. The film discs (with or without SBE) were applied on these
prepared surfaces. The plates with bacteria were incubated at 37 °C
for 24 h, with yeasts at 28 °C for 48 h, while those with fungi at 25 °C
for 72 h. After incubation, the diameter of growth inhibition around
the discs wasmeasured. The result was expressed in millimeters, without
subtracting the disc diameter (12 mm).