The isolate (AC001) of D. flagrans, a nematode-trapping fungus
belonging to the genus Duddingtonia, was kept in test tubes containing
2% corn-meal-agar (2% CMA), at 4 C, in the dark. The isolate
was obtained from a Brazilian soil using the soil sprinkling
method (Duddington, 1955), modified by Santos et al. (1991). Fungal
mycelia were obtained by transferring culture disks (5 mm in
diameter) of fungal isolates in 2% CMA to 250 mL Erlenmeyer flasks
with 150 mL liquid potato-dextrose medium (Difco), pH 6.5, and
incubated under agitation (120 rpm), in the dark at 26 C, fo