Abbreviation (EPA): Eicosapentaenoic acid; (DHA): Docosahexaenoic acid; (PUFA): Polyunsaturated fatty acids; (LC-PUFA): Long-chain polyunsaturated fatty acids; (RHLB): Required hydrophilic-lipophilic balance; (CG): Gas chro- matograph; (NEMROD): New Efficient Methodology for Research using Optimal Design; (HLB): Hydrophilic- lipophilic balance; (FAMEs): Fatty acid methyl esters; (DTS): Dispersion technology software; (RI): Refractive index; (DSC): Differential Scanning Calorimetry; (PdI): Polydispersity index; (NUT): Nephelometric Turbidity Units; (TG): triacylglycerols.