Few reviews have been published in the literature for the quantification of synthetic food dyes. Ahlström et al. (2005) have reviewed the development of analytical procedures for determination of banned azo dyes in consumer goods. Chung and Cerniglia (1992) have evaluated the mutagenicity effect of azo dyes by structure–activity relationship studies and found that biologically active dyes are mostly limited to those compounds having p-phenylenediamine and benzidine moieties. Kaur and Gupta (2012) have reviewed the determination of water soluble and water insoluble food dyes by spectrophotometry. Kucharska and Grabka (2010) reviewed chromatographic methods for the determination of synthetic dyes. LC-UV–Vis and LC–MS methods for analysis of Sudan I–IV dyes along with their extraction procedure in various food matrices were reviewed by Rebane, Leito, Yurchenko, and Herodes (2010). However, there is not even a single comprehensive review which clearly describes all the analytical methods which are generally used to determine the ADF in food products, which will be quite valuable for not only to the food analysts but also regulatory authorities for monitoring the quality and safety of food products. In order to fill this gap in the literature, the present manuscript describes the various methods used for analysis of ADF and their extraction from complex food matrices.