3. Results and discussion
The antimicrobial activities of soil, saliva and mixture of both
soil and saliva were tested against the three tested pathogenic
organisms. The results revealed that, soil alone showed moder-
ate antimicrobial activity compared to saliva which did not
produce any antagonistic effect. On the other hand, mixture
of soil and saliva showed the highest antimicrobial activity
(Fig. 1). The same trend was observed for the cell free bacterial
and fungal culture media. Thus, the pathogenic organisms
showed higher sensitivity to the filtrates resulted from mixing
soil and saliva. Based on the above mentioned results, all the
experiments were carried out using the mixture of soil and
saliva. This is may be due to that the alkalinity of saliva may
favour the growth of antibiotic producing microorganism.
Furthermore, saliva act as oral antiseptic because it contain