The main outcomes of this study can be summarized as
The data provided in this study pave the way for cost-effective
recombinant production of HRP isoenzymes in P. pastoris. Current
efforts are made in our lab to provide detailed information on
the identification of new HRP isoenzymes from a next generation
sequencing of the horseradish transcriptome and to show classifying
data on the new HRP isoenzyme sequences (Näätsaari et al., in
preparation). Future in-depth studies will provide information on
the molecular mechanisms underlying the differences in activity
and stability of the various interesting HRP isoenzymes.