4. Vegetable oil 5 mL + 0.50 ml Ferric chloride and 0.50 ml ascorbic acid (15 μM and
100 μM)
5. Vegetable oil 5 mL + 0.50 ml Ferric chloride, 0.50 ml ascorbic acid, EDTA (100 μM)
6. Vegetable oil 5 mL + 0.50 ml Ferric chloride, 0.50 ml ascorbic acid, BHA (100 μM) **
add BHA before prooxidant **
Allow the samples to incubate for 30 min at room temperature. Shake occasionally to
incorporate oxygen.